STUDIES ON THE PRODUCTION OF α- AMYLASE BY Bacillus subtilis

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Studies on the production of alkaline α-amylase from Bacillus subtilis CB-18.

BACKGROUND Amylases are among the main enzymes used in food and other industries. They hydrolyse starch molecules into polymers composing glucose units. Amylases have potential applications in a number of industrial processes including foods and pharmaceutical industries. Alkaline α-amylase has the potential of hydrolysing starch under alkaline pH and is useful in the starch and textile industr...

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Influence of media composition on the production of alkaline α-amylase from Bacillus subtilis CB-18.

BACKGROUND Starch, a homopolysaccharide is an important and an abundant food reserve and energy source. Starches are processed to yield different products which find many industrial applications. Alpha-amylases hydrolyze starch by cleaving α-1,4-glucosidic bonds and have been used in food, textile and pharmaceutical industries [Sun et al. 2010]. Enzymatic conversion of starch with amylase prese...

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Production, purifi cation, and characterization of α-amylase by Bacillus subtilis and its mutant derivates

Th e eff ects of various carbon and nitrogen sources on production of α-amylase by Bacillus subtilis and its mutant derivates were investigated. Th e maximum production of α-amylase by all strains was obtained in the presence of mesoinositol as the carbon source. Th ere was no more signifi cant increase in enzyme yield in the case of the supplementation of nitrogen sources, whereas malt extract...

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PRODUCTION OF a-Amylase FROM BANANA PEEL BY Bacillus subtilis

We produced a-amylase from banana peel by Bacillus subtilis through solid state fermentation (SSF). Addition of yeast extract, corn steep liquor, sodium dodesyl sulphate (SDS) and Tween-80 enhanced the a-amylase production under preoptimized fermentation conditions. Maximum enzyme yield (10.25 U/mL) was observed at 35°C after 24 hours of SSF in growth medium containing 0.1% yeast extract, 1% co...

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A segregated model for heterologous amylase production by Bacillus subtilis

A segregated model was proposed to investigate the inherent relationships between growth, substrate consumption, cell differentiation and product formation in a Bacillus subtilis fermentation producing heterologous amylase in a 22-l bioreactor. The segregated model includes three distinguishable cell states and the transition from vegetative phase to sporangium and finally to mature spore. An a...

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ژورنال

عنوان ژورنال: IOSR Journal of Engineering

سال: 2012

ISSN: 2278-8719,2250-3021

DOI: 10.9790/3021-020510531055